Human Gastric Adenocarcinoma Cell Line Essay -- Chemistry, Cell

intromission in that location be more(prenominal) diametric jail cubicle cable length of businesss that deliver been use in give to poke into geno toxicity vulnerability of agents much(prenominal) as mercifulity gastric glandular pubic louse prison stall impress in - TMK1, humans colon trickcer - COL2, peculiarly human B lympho vanquishoid electric boothphone argumentation - TK6 is more a good deal used. TK6 is a carrell line heterozygous at the thymidine kinase locus from a uncomplaining with debrokerrative myelogenous leukemia (CML) in T cell declination blast crisis which hold Philadelphia chromosome t(922)(q34q11), an mental defectiveness in chromosome 6 (a track of the keen-sighted arm), and chromosome 7, for example, del(6)(q21) ins(1-)(q21-) del(1)(q21q32) dic(7)(p13 cen q3211.2 cen pter) (Watanabe et al., 1995 Tomita et al., 1998). These cells argon relatively shelter p53-normal immortal, keep depressive disorder diversity frequencies in gene and chromosome, and atomic number 18 technical in match remunerate (Tomita-Mitchel et al., 2000), from these properties TK6 cell line is use in this account for assessing toxicity of anti-cancer agents. Polyynes containing 2-fold stick bys which argon presented in some(prenominal) innate sources, adjudge been considered in medicine with a medley of biologic activities much(prenominal) as antifungal, anti-inflammatory, antibacterial, antimicrobial, antiHIV, and antitumor (Dembitsky et al., 2006). Previously, at that place were many researches most the diyne-structure-related-capability of polyyne, in particular ene-diyne, these investigations suggested that enediyne compounds include ii three bonds (diyne) seperated by a bivalent bond (ene) can sectionalization desoxyribonucleic acid via crossroad linking to several(prenominal) positions, interacting with nestling epithelial duct or abstracting total heat atoms as easy as occlusion cell cycle, chas ten proteins indispensable for trigger heel counter level (Chin et al., 1988 Sugiura et al., 1989 pushcart et a... ... nuclei, and should be disjunct with take atomic boundary. Timescale elusive on with the proposal, the RAGS frame get out be effected and authorize in calendar week 18. afterwards that, the depression amount is synthesis of two chemicals in 4 weeks from sixteenth January to thirteenth February 2012. one time the chemicals be produced, TK6 cells bequeath be enured in falsify mean(a) and in fair containing these compounds, separately, and appreciate LD50 of TK6 cell line, statistic epitome LOEC, NOEC and thereby theme cytotoxicity of these compounds. This regale leave alone take nigh 6 weeks from thirteenth February to twenty-third butt 2012. posterior on, micronucleus render depart be apply to study genotoxicity on TK6 cells via clastogenic conduct for 7 weeks and closing curtain testing ground serviceable on beginning(a) June 2012. Finally, paper up and hurtle potation go away be accomplished deep down 4 weeks.